IHTM PLAVI
University of Belgrade
Institute of Chemistry, Technology and Metallurgy
National Institute of the Republic of Serbia

grb BUUniversity of Belgrade

Nataša Božić, Ph.D.

Senior research assosiate
Staff scientist

 

 

 

 

 

Address:

IHTM, Centar za hemiju, Studentski trg 12-16, 11000 Beograd
11158 Beograd 118, PAK 105104
Laboratorija 483, I sprat
Telephone: 011 32 82 393
Fax: 011 26 36 061
E-mail: nbozic@chem.bg.ac.rs

Education:

2000 Bachelor of Biochemistry, Faculty of Chemistry, University of Belgrade.
2003 Master of Biochemistry, Faculty of Chemistry, University of Belgrade.
2007 Ph.D. in Biochemistry, Faculty of Chemistry, University of Belgrade.
2009 Post-doctoral research study in the field of fermentation technology, Department of Chemical Engineering, Autonomous University of Barcelona.
2010, 2011, 2012 Research studies in the field of molecular biotechnology, Department of Chemical Engineering, Autonomous University of Barcelona.

Research Ranks:

2003 Research Trainee
2004 Research Assistant
2008 Research Associate
2012 Senior Research Associate

Memberships:

Serbian Chemical Society
Serbian Biochemical Society
FEBS
European Federation of Biotechnology

Professional Experience:

2000-2003 - Faculty of Chemistry, University of Belgrade.
2003 - ICTM, Department of Chemistry, University of Belgrade.

Awards and Honors:

2009 Postdoctoral Scholarship of the Ministry of Science and Technological Development of the Republic of Serbia.
2010- FEBS Fellowship (Federation of European Biochemical Societies).
2010 Medal of the Serbian Chemical Society for enthusiasm and achievement in science.

Research Interests:

Upstream and downstream processing of glucohydrolases. Protein engineering, immobilization and application of carbohydrate (starch)-active enzymes. Establishing the relationships between the structure of carbohydrate (starch)-active enzymes and their precise specificity. Exploring application potential in various areas, from the search of novel enzymes for biofuel production to the exploration of the gut microbiota.

Foreign languages: English

Projects:
International:
2011 - 2013 Production, characterization and use of enzymes in biocatalysis: Hydrolases and oxidoreductases, Serbian-Spanish action.
2012 - 2015 Production and application of α-amylase from soil Bacillus strains, CRP - ICGEB Research project.
National:
2003 - 2005 Natural products from plants and marine organisms: Medical and ecological significance.
2006 - 2010 Interaction of natural products and their analogs with proteins and nucleic acids.
2011 - Production, isolation and characterization of the enzyme in the soluble and immobilized form in food biotechnology, biofuels and environmental protection.

Publications:

Published papers:

  1. Lončar, N., Slavić, M.Š., Vujčić, Z., Božić, N. Mixed-mode resins: Taking shortcut in downstream processing of raw-starch digesting α-amylases, Scientific Reports, 2015 5:15772, DOI: 10.1038/srep15772.
  2. Šokarda Slavić, M., Pešić, M., Vujčić, Z., Božić, N. Overcoming hydrolysis of raw corn starch under industrial conditions with Bacillus licheniformis ATCC 9945a α-amylase, Applied Microbiology and Biotechnology 2015, in press, DOI 10.1007/s00253-015-7101-4.
  3. Vujčić, Z., Janović, B., Lončar, N., Margetić, A., Božić, N., Dojnov, B., Vujčić, M.  Exploitation of neglected horseradish peroxidase izoenzymes for dye decolorization, International Biodeterioration and Biodegradation, (2015) 97, 124-127.
  4. Gligorijević, N., Stevanović, N., Lončar, N., Baošić, R., Vujčić, Z., Božić, N. A thin layer chromatographic comparison of raw and soluble starch hydrolysis patterns of some α-amylases from Bacillus sp. isolated in Serbia, Journal of the Serbian Chemical Society, (2014) 79, 411-420.
  5. Pešić, M., Božić, N., López, C., Lončar, N., Álvaro, G., Vujčić, Z. Chemical modification of chloroperoxidase for enhanced stability and activity, Process Biochemistry, (2014) 49, 1472-1479.
  6. Lončar, N., Gligorijević, N., Božić, N., Vujčić, Z. Congo red degrading laccases from Bacillus amyloliquefaciens strains isolated from salt spring in Serbia, International Biodeterioration and Biodegradation, (2014) 91, 18-23.
  7. Slavić, M.S., Božić, N., Vujčić, Z. Growth temperature of different local isolates of Bacillus sp. in the solid state affects production of raw starch digesting amylases, Archives of Biological Sciences, (2014) 66, 483-490.
  8. Božić, N., Slavić, M.Š., Gavrilović, A., Vujčić, Z. Production of raw-starch-digesting α-amylase isoform from Bacillus sp. under solid-state fermentation and biochemical characterization, Bioprocess and Biosystems Engineering, (2014) 37, 1353-1360.
  9. Lončar, N., Božić, N., Lopez-Santin, J., Vujčić, Z. Bacillus amyloliquefaciens laccase - from soil bacteria to recombinant enzyme for wastewater decolorization, Bioresource Technology  (2013) 147, 177-183.
  10. Božić, N., Puertas, J.M., Lončar, N., Duran, C.S., Lopez-Santin, J., Vujčić, Z. The DsbA signal peptide-mediated secretion of a highly efficient raw-starch-digesting, recombinant alpha-amylase from Bacillus licheniformis ATCC 9945a, Process Biochemistry, (2013) 48, 438-442.
  11. Dojnov, B., Pavlović, R.,  Božić, N.,  Margetić, A., Nenadović V., Ivanović J., Vujčić Z. Expression and distribution of cellulase, amylase and peptidase isoforms along the midgut of Morimus funereus L.(Coleoptera: Cerambycidae) larvae is dependent on nutrient substrate composition, Comparative Biochemistry and Physiology Part B (2013) 164, 259–267.
  12. Popović, V., Atanasijević, T., Nikolić,  S., Božić, N., Vujčić,  Z., Micić-Labudović,  J. Forensic aspects of postmortem serum carbohydrate-deficient transferrin analysis as a marker of alcohol abuse. Srpski Arhiv za Celokupno Lekarstvo, (2013) 141, 203-206.
  13. Dojnov, B., Vujčić, Z., Božić, N., Margetić, A., Vujčić, M., Nenadović, V., Ivanović, J. Adaptations to captive breeding of the longhorn beetle Morimus funereus (Coleoptera: Cerambycidae); application on amylase study. Journal of Insect Conservation (2012) 16(2), 239-247.
  14. Božić, N., Ruiz, J., López-Santín, J., Vujčić, Z. Optimization of the growth and α-amylase production of Bacillus subtilis IP 5832 in shake flask and laboratory fermenter batch cultures. Journal of the Serbian Chemical Society (2011) 76(7), 965-972.
  15. Vujčić, Z., Miloradović, Z., Milovanović, A., Božić, N. Cell wall invertase immobilisation within gelatin gel. Food Chemistry (2011) 126(1), 236-240.
  16. Lončar, N., Božić, N., Andelković, I., Milovanović, A., Dojnov, B., Vujčić, M., Roglić, G., Vujčić, Z. Removal of aqueous phenol and phenol derivatives by immobilized potato polyphenol oxidase. Journal of the Serbian Chemical Society (2011) 76(4), 513-522.
  17. Božić, N., Ruiz, J., López-Santín, J., Vujčić, Z. Production and properties of the highly efficient raw starch digesting α-amylase from a Bacillus licheniformis ATCC 9945a. Biochemical Engineering Journal  (2011) 53(2), 203-209.
  18. Vujčić, Z., Lončar, N., Dojnov, B., Milovanović, A., Vujčić, M., Božić, N. Characterisation of leucyl aminopeptidase from Solanum tuberosum tuber. Food Chemistry (2010) 121, 418-423.
  19. Lončar, N., and Vujčić, Z., Božić, N., Ivanović, J., and Nenadović, V. Purification and properties of trypsin-like enzyme from the midgut of Morimus funereus (Coleoptera, Cerambycidae) larvae. Archives of Insect Biochemistry and Physiology (2010) 74(4), 232-246.
  20. Vujčić, Z., Milovanović, A., Božić, N., Dojnov, B., Vujčić, M., Andjelković, U., Lončar, N. Immobilization of cell wall invertase modified with glutaraldehyde for continuous production of invert sugar. Journal of Agricultural and Food Chemistry (2010) 58(22), 11896–11900.
  21. Dojnov, B., Lončar, N., Božić, N., Nenadović, V., Ivanović, J., Vujčić, Z. Comparison of α-amylase isoforms from the midgut of Cerambyx cerdo L.(Coleoptera: Cerambycidae) larvae developed in the wild and on an artificial diet. Archives of Biological Science (2010) 62(3), 575-583.
  22. Lončar, N., Božić, N., Nenadović, V., Ivanović, J., Vujčić, Z. Characterization of trypsin like enzymes drom the midgut of Morimus funereus (Coleoptera: Cerambycidae) larvae.  Archives of Bioligal Science (2009) 61(4), 713-718.
  23. Božić, N., Ivanović, J., Nenadović, V., Bergstrom, J., Larsson, T., Vujčić, Z. Purification and properties of major midgut leucyl aminopeptidase of Morimus funereus (Coleoptera, Cerambycidae) larvae. Comparative Biochemistry and Physiology (2008) 149B, 454–462.
  24. Dojnov, B., Božić, N., Nenadović, V., Ivanović, J., Vujčić, Z. Purification and properties of midgut α-amylase isolated from Morimus funereus (Coleoptera: Cerambycidae) larvae. Comparative Biochemistry and Physiology (2008) 149B, 153-160.
  25. Božić, N., Dojnov, B., Milovanović, A., Nenadović, V., Ivanović, J., Vujčić, Z. Characterization of endopeptidases from the midgut of Morimus funereus (Coleoptera: Cerambycidae) larvae, Archives of Biological Sciences Belgrade (2008) 60 (3), 403-409.
  26. Milovanović, A., Božić, N., Vujčić, Z. Cell wall invertase immobilization within calcium alginate beads. Food Chemistry (2007) 104(1), 81-86.
  27. Dojnov, B., Božić, N., Bulajić, N.,Vujčić, Z. Preparation of combined extract of cell wall and cytosol antigens of Candida albicans for immunoblot analysis. Journal of Clinical Laboratory Analysis (2007) 21, 406-412.
  28. Radulović, Ž., Milutinović, M., Anđelković, M., Vujčić, Z., Tomanović, S., Božić, N., Marinković, D. Allozyme polymorphism of Mdh and α-Gpdh in Ixodes ricinus populations: comparison of borreliae-infected and uninfected ticks. Experimental and Applied Acarology (2006) 40(2), 113-121.
  29. Božić, N., Vujčić, Z. Detection and quantification of leucyl aminopeptidase after native electrophoresis using leucine-p-nitroanilide. Electrophoresis (2005) 26(12), 2476-2480.
  30. Božić N; Vujčić Z; Ivanović J; Nenadović V. Isoforms of leucyl-aminopeptidase of Cerambyx cerdo (Coleoptera, Cerambycidae) larvae. Archive of Biological Sciencies Belgrade (2004) 56, 1P-2P.
  31. Sladić, D., Novaković, I., Vujčić, Z., Božić, T., Božić, N., Milić, D., Šolaja, B., Gašić, M.J. Protein covalent modification by biologically active quinones. Journal of the Serbian Chemical Society (2004) 69(11), 901-907.
  32. Božić, N., Vujčić, Z., Nenadović, V., Ivanović, J. Partial purification and characterization of midgut leucyl aminopeptidase of Morimus funereus (Coleoptera: Cerambycidae) larvae. Comparative Biochemistry and Physiology (2003) 134B(2), 231-241.
  33. 33. Novaković, I., Vujčić, Z., Božić, T., Božić, N., Milosavić, N., Sladić, D. Chemical modification of α-lactoglobulin by quinones. Journal of the Serbian Chemical Society (2003)  68(4-5), 243-248.